DDW Suppresses Growth of Cancer Cells
The DDW effect on proliferation of human breast adenocarcinoma MCF7, human lung carcinoma A549 and human colorectal adenocarcinoma HT29 cells was assessed. When the cells were grown for 48 h in DDW with deuterium concentration of 60 ppm, 80 ppm, 100 ppm, and 120 ppm and in normal water (NW, 150 ppm D) as a control, MCF7 cells responded weakly to DDW treatment, whereas HT29 cells showed a ≈20% reduction in proliferation at 100 ppm D. The A549 cells turned out to be most responsive to DDW (Fig. 2A), with 80 ppm DDW inhibiting cell proliferation by 32% (p < 0.005). Therefore, the A549 cells were selected for further proteomics studies. At both lower and higher D concentrations compared with maximum suppression, cell proliferation restored to normal levels